2359. Prospective Feasibility Study for Novel Ultrasensitive Multiplexed Immunoassay for Clostridiodes difficile Toxins A and B
نویسندگان
چکیده
منابع مشابه
An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins.
We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h . It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C. difficile toxins in the fecal and serum specimens of experime...
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The toxins produced by Clostridium difficile share several functional properties with other bacterial toxins, like the heat-labile enterotoxin of Escherichia coli and cholera toxin. However, functional and structural differences also exist. Like cholera toxin, their main target is the disruption of the microfilaments in the cell. However, since these effects are not reversible, as found with ch...
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A Rapid Enzyme Immunoassay for the Detection of Clostridium difficile Toxins A and B in Stool Specimens
AND EXPLANATION OF THE TEST Toxigenic Clostridium difficile is the leading cause of nosocomial infectious diarrhea in developed countries. An estimated 300,000 cases of C. difficile associated disease (CDAD) are seen per year in U.S. hospitals alone. 2 Clostridium difficile is the etiologic agent in approximately 25% of all cases of antibiotic-associated diarrhea. Virtually any antibiotic can p...
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ژورنال
عنوان ژورنال: Open Forum Infectious Diseases
سال: 2019
ISSN: 2328-8957
DOI: 10.1093/ofid/ofz360.2037